Published by the china pharmaceutical industry association, yunnan province
T/ynzyc0108-2025
Foreword
This document is in accordance with the provisions of part 1 of the standardized working guidelines gb/t1. 1-2020: the structure and drafting rules for standardized documents
Done。
Please note that certain elements of this document may relate to patents and that the issuer of this document does not have the responsibility to identify patents。
This document was presented and concluded by the china pharmaceutical industry association of yunnan province。
The present document was prepared by the college of technology, the kunming polytechnic university, the association of the chinese pharmaceutical industry of yunnan province, yunnan sen ho bacillus industries limited, inc
Zhao tong city academy of tianma。
The main drafters of this document are yang sung-chang, cheng liang, choi su-ming, yang nok, soo, chen xiaohong, shin kai-sawa, tong peng, ho hai-ying, yang
Cheng jie, han do, zhang young-suk, ding ming, lee shibo, mu jia yun。
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Technical protocol for the production of honey circulus for the production of green pharmacist molybdenum
1 scope
This document sets out the technical requirements, production specifications, labels, packaging, transport and storage of honey circulus, which is applicable to yunnan's zhuuntung zhongmae cultivation
Save。
The present document applies to the production of green and standardized honey circulus for the cultivation of zhutun。
2 normative citation documents
The content of the following documents constitutes an essential provision of this document through their normative references. Of which, the date of reference
Only the version corresponding to that date applies to this document; date-free references, the latest version (including all change orders) apply to this document
Documentation。
Gb4789. 28 national standard food microbiology testing quality requirements for nutrients and reagents for food safety
Gb4806. 1 national standard food exposure materials and products general safety requirements for food safety
Gb5749 standard of drinking water sanitation
Gb/t191 packaging storage icon
Gb/t6543 packaging in single and double-walled boxes
Gb/t12728 edible fungi terminology
Gb/t41220 composite plastic covering for food packaging
Ny/t1935 quality safety requirements for edible bacterial culture matrix
Ny/t2375 technical guidelines for the production of edible bacteria
3 terminology and definitions
The following terms and definitions apply to this document。
3. 1
Utima
Yunnan's natural plant, gastrodiaelata bl., all of which is gray, plastered and bouquets, and many of which are wide-breeded and eggs
Shape, ellipse。
3. 2
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Meekles
A symbiotic fungus that ensures the normal growth of the tama, a fine nectar ring of the comyote parasol, armillariellamella (vahl. Ex)
Karst。
3. 3
Locked combination
A specific form of bi-nucleus filamental formation, often at the top of the membrane, starts with a cell bulge
Up, bend down, connect to the lower cell, like a lock. Locking combinations are the criteria for the identification of bi-nucleus
Mybs can be identified as binuclastic. The process of forming a lock-like union ensures that the bi-nucleus is divided in each section (per cell)
They can all contain two different (genetic) types of nuclear, laying the foundation for sexual reproduction and the formation of a burden through nuclear assembly。
4 technical requirements
4. 1 quality requirements for bacterial species
4. 1. 1 parent species
The provisions of table 1 should be met。
Table 1 quality requirements for mother species of honey circulus
Project requirements
Using glass test tubes, typically 18 mm x 180 mm or 20 mm x 200 mm, shall be assured
Containers
It's intact。
Packagings covered with silicone or cotton plugs, dry, clean, loose and moderate to meet the requirements of air penetration and filtering
Injection of culture base is one half to two thirds of total test tube accumulation
Top piping plug 40mm-50mm
Vaccination (size of vaccination block) (3-5) mm (3-5) mm
Sensor
Mybs grow to grow with culture
Request
Chromic cords formed from a muscular muscular muscular characterization base with white cords in the culture base
The bacterial fluorescent filaments and the juvenile cords give green and white fluorescent in the dark. Light
Spectrum filamental surfaces flat, flat, non-aging
There's a small amount of brown matter in the fungus
There's no fungus
On the back side of the slopes, the appearance of the culture base is dry, the colours are even, and there are no shadows
It smells like honey circulus
Mybs grow in big, full, even
Microorganisms
It's a joint lock
Learning requirements
No fungus
4. 1. 2 original
The provisions of table 2 should be met。
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Quality requirements for primary species of honey circulus
Project requirements
Using glass or plastic bottles with a volume of 500 ml, resistant to high temperatures of 126°c, light or colourless
< 4 cm in diameter of the container vessel; the packaging covers with or without cotton plastic plugs; the container and container covers shall end
It's intact。
Packagings are dry, clean, loose and moderate to meet air and filter requirements
• 50 mm ± 5 mm above the container at the surface distance of the culturer input culture
Vaccinations (number per mother, vaccinations)
X
(4-6) bottle, 12mm15mm
Sensory size)
We're going to need a lot of growth
Chromic cords formed from a muscular muscular muscular characterization base with white cords in the culture base
The fluorescent filament and the juvenile cord emit green and white fluorescent in the dark. Light
Bacteria
All parts of the fungus are growing evenly and without aging
Appearance
The culturer and the fungus have a small amount of brown matter that can be colored. Deeper
There's no fungus
There's no fungus
It smells like honey circulus
Mybs grow in big, full, even
Microorganisms
It's a joint lock
Learning requirements
No fungus
4. 1. 3 cultivation plant
The provisions of table 3 should be met。
Table 3 quality requirements for primary species of honey circulus
Project requirements
Packagings are not less than 14 cm x 28 cm plastic bag, with an air hole in the middle, gypsy。
Packaging plastic bags shall conform to the provisions of gb/t41220, and the packaging covers shall be used to provide for ablution and
The plastic cover required for the filter。
Packagings are dry, clean, loose and moderate to meet air and filter requirements
• 50 mm ± 5 mm above the container at the surface distance of the culturer input culture
Vaccinations (number of seed plants per bottle) (4-6) bottles, ≥12 mm x 15 mm
Sensory filaments grow with culture
We're going to have to develop a brown chromosomal cord that's sprouted from the outside, and a white chromium
Microfilamental characteristics
The bacterial fluorescent filaments and the juvenile cords give green and white fluorescent in the dark. Light
The appearance of different parts of the fungus grows evenly and without aging
The culturer and the fungus have a small amount of brown matter that can be colored. Deeper
There's no fungus
There's no fungus
It smells like honey circulus
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Project requirements
Microbacteria are growing in a strong, full and even state
It's called for a lock form
No fungus
4. 2 cultivation site layout and facilities
4. 2. 1 plant layout and construction
The area of production should be strictly separated from the raw material bank, the finished stock and the living area, in accordance with site characteristics and production requirements. Production area
Independently isolated tanshows, distribution rooms, sterilization rooms, cooling rooms, vaccination rooms, culture rooms, storage rooms, bacterial testing rooms, etc., and reasonable
Arrangements are made to meet the requirements of environmental conditions at different stages of the growth of the meringue. Human flow is separated from logistics and bacterial and sterile areas are isolated。
The sunstand should be flat, without shelter and far from the source of the fire. There should be sufficient space in the assembly rooms to ensure ease of water and electricity. The sterilization room should ensure that..
Electricity and water are installed in a reasonable manner, with a smooth schedule and easy access. The cooling room should be clean and dust-proof and free of heat. The inoculation room should be equipped with a buffer room
The gas equipment, interior walls and roofs are smooth and frequently cleaned and disinfected。
4. 2. 2 facilities
The size of the facilities in the production chain should be matched. The cooling room, inoculation room, culture room and storage room are provided with heating facilities。
Should be equipped with blenders, bottled (bags)
