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  • Experimental step on ph for enzyme activity

       2026-04-04 NetworkingName540
    Key Point:The purpose of the 1st experiment was to explore the effects of ph on the activity of the hydroxylase peroxide by adding a 3% volume peroxide solution in the 2 ml new formulation in the 1st, 2nd and 3rd tubes, then adding l ml distillation to the 1st tube, adding 1 ml (equal) sodium hydroxide solution to the 2nd tube, adding 1 ml (equal) hydrochloric acid solution to the 3rd tube and oscillating test tubes。2 the analysis of the subject is

    The purpose of the 1st experiment was to explore the effects of ph on the activity of the hydroxylase peroxide by adding a 3% volume peroxide solution in the 2 ml new formulation in the 1st, 2nd and 3rd tubes, then adding l ml distillation to the 1st tube, adding 1 ml (equal) sodium hydroxide solution to the 2nd tube, adding 1 ml (equal) hydrochloric acid solution to the 3rd tube and oscillating test tubes。

    2 the analysis of the subject is known to be the drop of 2 drops of grinding fluid in the liver of chickens in test tubes 1, 2 and 3。

    The amount of bubble 3 reflects the high and low activity of the peroxide hydroase. See carefully how much bubbles are generated in the test tubes and record them。

    The activity of the enzyme is highest when ph is best; when above or below ph, the activity of the enzyme is reduced. The result of the experiment was that more bubbles were produced in test tubes 1 and less in test tubes 2 and 3。

    In addition to ph, there are other environmental factors that affect the activity of the hydroxygen enzyme peroxide, for which a study is proposed on the effects of temperature on the activity of the hydroxygen peroxide。

    So the answer is:

    11 ml (equivalent) sodium hydroxide solution (test dose and reagent name missing no score) 1 ml (equivalent) hydrochloric acid solution (test dose missing no score)。

    2 chicken liver grinder

    3 produces more bubbles

    Explore the effect of temperature on hydroxyl oxide activity (or other reasonable answer)

    Point assessment: the examination of the factors affecting enzyme activity and the exploration of experiments focuses on the detection of experiments affecting enzyme activity, requiring students to master the principles of experimental design and to determine accurately the self-variant, causal and non-independent variables of the experiment。

    At the same time, the factors that influence enzyme reaction rates need to be understood. As a biological catalyst, enzyme is characterized not only by general inorganic catalysts, but also by specificity, efficiency and moderation, all of which are good materials for biological experiments... With enzyme characteristics as the background of knowledge, which allow better testing of students ' experimental knowledge and operational abilities。

     
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