T/mscool-2023
China bacteria society group standards
T/mscool-2023
High-altitude ooze cultivation: technical protocols for the production of fungi
[referral draft]
2023-*-* publication
2023-*-* implementation
Published by the china bacteria society
T/mscool-2023
Contents
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2 normative bow i file 2
3 terms and definitions 2
4 technical requirements 3
Use of 5 fungi species 6
Foreword
This standard is prepared in accordance with the provisions of gb/t1. 1-2009, part 1 of the standardized working guidelines: structure and preparation of standards
Make。
This criterion is referred to by the chinese bacteria society。
The standard was drafted by the institute of pharmaceutical plants of the chinese academy of medical sciences。
The principal drafter of this standard, guo sun-sung, zeng xue, hae-hin
Date of first release of this standard
1 scope
This standard sets out the requirements, production techniques, etc. For the embryonic strains used in high-altitude ooze cultivation。
2 normative citation documents
The following documents are essential for the application of this document. References to all date notes, version of date notes only
This document is applicable in thousands. The latest version (including all amendments) of an undated reference document applies to this document。
Gb/t12728 edible fungi terminology
Ny/t528 technical protocol for the production of edible bacterial species
Ny/t1935 quality safety requirements for edible bacterial culture matrix
Db51/t1640 mit protocol
3 terminology and definitions
3. 1 high altitude obituary
Gastrodiaelata bl. F. Glaucas. Chow, a variant of tima, brown, white even
Stripes, blue and green flowers, high detachment rate, excellent species, and production in mountain areas at 1,200-2000 metres above sea level
Utima, good quality, good taste, high altitude。
3. 2 epidemics
A symbiotic fungi that provides basic nutrients for the growth of obituary seeds, a part of the fungi strain of the obscurinium mushrooms
It's like a rocky mushroom。
3. 3 bacteria
Precious fungus filament cultures used in reproduction or cultivation, including mother species, primary species and plant varieties。
The mother stockculture selects, in various ways, the pure culture of the filamental bacteria and its successors
Things. It's also called a level one, a test tube。
Gb/t12728-2006, definition 2. 5. 7
The motherspahn is a pure culture of bacterial filaments developed by mother-plant transplants. Also known as level 2。
Gb/t
12728-2006, definition 2. 5. 8
Cultivated planting spawn: pure culture of fungus filaments from original transplantation and expansion. It's also called level 3。
Gb/t12728-2006, definition 2. 5. 9
3. 4 bacillus examination
A strain suitable for high-altitude ooze cultivation should be selected, seeded from units with corresponding qualifications and of a clear caste. Oh, no
Organismal separations with unknown, undefined species and productive form not tested systematically should be used as a source of seed production
Bacteria。
The bacterial mother should be tested in terms of the quality and genre of the bacteria, including purity, before the species enter the expanded production process every year
Vibrant, mucous - mutagenic, velocity of mycous growth, appearance, etc。
3. 5 substrates
Provide growing breeding materials for bacterial growth. The culture base shall be in accordance with ny/f1935 and shall not be selected for deterioration, pollution
Raw materials should not be added to pesticides, fertilizers, etc。
The matrix formulations include natural materials such as shell-cracking leaves, fruit trees or broad leaves, or wood crumbs, barley, corn cores, etc
Require fresh, insect-free, impurity, clean, dry; nutritional supplements such as glucose, magnesium sulphate, potassium dihydrogen phosphate, etc
Chemical purity and above。
The following table is recommended for the formulation of bacteria at all levels:
Table 1 recommended matrix formulation for bacterial species
I don't know,
Bacillus name stock level matrix formulation^/
200g for leather organic potatoes, 20g for white sugar, 20g for joan. Pass
Mother
Add ion water to 1,000 ml. Heart
(1) 50% wood crumbs, 29. 7% cotton seed shell, 20% wheat, 0. 1% magnesium sulfate, phosphorus
Potassium acid dihydrogen 0. 2 per cent with a water content of about 65 per cent。
Original type (2) wooden stickers (24-hour immersion), filled with fine wood crumbs totalling 50 per cent, wheat
Microbacteria 49. 7 per cent, magnesium sulfate 0. 1 per cent, potassium dihydrophosphate 0. 2 per cent and approximately 65 per cent water。
(3) 30% wheat, 70% wood crumb and about 65% water。
, (1) fragmented leaves (impregnated 24h) 50% and wheat and wood crumbs (v:v=l 1) 50%,
Mixing leaves。
Cultivation
(2) wheat 30%, rough maize core 50%, rough wood crumb 19. 7%, magnesium sulfate 0. 1%,
Jen zao
Potassium dihydrophosphate, 0. 2%, water content, 65%。
4 technical requirements
Bacteria production shall be in accordance with the requirements of ny/f528。
4. 1 technical staff
There should be technicians, including operators and inspectors, required for the production of bacterial species。
4. 2 site requirements
The terrain is high, ventilation is good, drainage is good and transport is easy。
4. 3 health requirements
Plant installations should be kept clean and clean, with no poultry or livestock in 300m, no garbage (septic) fields, no sewage and other contamination
