Guangzhou's scientists have been closing for five years to develop simple and efficient new methods
Inducing multi-energy stem cells can help humans understand the mystery of cell “diversion” and provide a window for the scientific community to look into the nature of life. Multi-energy stem cells can also be used to regenerate new tissues and organs, providing “seed” cell sources for disease treatment and regenerative medicine. Japanese scientists, shinya yamayama, who won the 2012 nobel prize for physiology or medicine, used viral vectors for genetic transport, with potential cancer risks and greater risks for subsequent clinical applications。
In order to induce body cells to become multi-energy stem cells, scientists in various countries are constantly developing new methods. At a later stage, scientists used chemical micromolecular substitutes for the multi-energy stem cells in the mountains, but there were shortcomings such as the number of steps, the length of time, the inefficiency and the lack of clarity。
After five years of work, the team of scientists led by mr. Pei has developed an efficient and simple method of semantic small molecules to induce multienergy stem cells, known as the cip (chemical industries of culture), which is the multifunctionality of compounds。
According to pei tei, the programme requires only two different “waters” for cells to “baptize” in order to “return to childhood” to stem cell status. This method is simpler and more efficient than previous programmes and requires fewer initial cells. More importantly, multiple body-cell types of “return to old age” can be achieved, including in vitro hepatocellular cells that are extremely difficult to cultivate。
The magic potion returns cells to their original state
How do these magical “magic potions” induce adult cells into the state of the embryo's multi-energy stem cells at an early stage of their development? Mr. Pei said that all cells in an individual have the same dye, and why do they have different forms and functions? Originally, before identifiable morphological changes occurred, cells were divided in a specific direction by being bound to determine their future developmental fate. Team studies have found that the fate of cells is controlled by the status of the "information hub" dyes within the cell's nuclear core. The chemical small molecules found by the research team induce multi-energy stem cell methods to modify genetic expression by directly integrating the small molecule, brdu, into dna and reshaping the dye structure, a completely new mechanism。
In this process, it is understood that two different types of “composed cocktail” (i. E. Liquid from a combination of compounds) need to be immersed in cells, with the first “composed cocktail” having a immersion time of about 16-24 days and the second “composed cocktail” having a immersion time of about 10-15 days. In the absence of the introduction of an external gene, the method is simple to operate, the conditions of the induction process are uniform, all components are clear and standardized. It will provide a safe and efficient preparation method for stem cell applications, with broad market applications prospects. At the same time, it provides a new direction for the development of drugs to induce the fate of cells, which will greatly contribute to the development of stem cells and regenerative medicine in the service of health care and hygiene in our country。
Xiehin, researcher at the shanghai institute of medicine and deputy director of the national new medicine screening centre at the central college of sciences, stated that the study had greatly improved the efficiency of induction and that chemical induction was expected to become a routine method for inducing multi-energy stem cells. This mechanism could also guide scientists in the targeted design of small molecules of compounds to change dye structure, thereby optimizing chemical induction and reprogramming systems. More importantly, chinese scientists complement each other in the field of compound inducers of multi-energy stem cells, putting our country at the forefront of the world in this field。
The co-author of the paper was the researcher and the researcher liu jing. Dr. Cao sang-tao, dr. Li dong wei and master yu sung yong were co-authors of thesis. The research is supported by funding from the national research and development centre, the institute of secondary education, the national fund for natural sciences, guangdong province and guangzhou city。
