Poster journalist, zhang zhang zanan
When it comes to pathological slices, most people start thinking about biology in secondary schools and observe the use of teaching tissues. Through those slices, we learn how to distinguish animal cells from plant cells and understand their structural characteristics. Pathological slices, on the other hand, are those specifically made of human pathologies that are often used to assist in the diagnosis of pathological conditions of patients on tumours。
Take a certain size of pathological tissue, wrap it in paraffins, slice it into thin slices, then dye it, and then further examine it and diagnose it with microscopes, which is the daily work of pathology。
In recent days, poster journalists have walked into the pathology section of the central district of the provincial hospital attached to shandong first medical university (shandong provincial hospital), closely observing, learning about the “birth” of pathological slices and the work of pathologists。
The material room is the first stop for the pathological organization. Here, doctors cut tissue blocks with a width of 1-2 cm, depending on the region and nature of the disease, and fix them to form tissue specimens. This is the “basic work” of every pathologist, who has told journalists that they need at least a dozen examples of tissues in the pick-up room in order to carry out their next studies。
Doctors are cutting the tissue in the material room
When the tissue block reaches the dehydration chamber, the doctor will dehydrate and replace the moisture within the tissue block, depending on the circumstances. The pathology section of the shandong provincial hospital has rapid dehydration techniques, which take only two hours, greatly increasing the speed of the issuance of pathological reports and reducing the waiting time for patients and their families。
The burial room is where the wax is immersed and buried. Using liquid paraffins to transform dehydrated tissues into wax tissue, they are sent to the technical room for slices and stickers。
The slicers are using a slicer to cut out the tissue of micrometre thickness
In the technical room, which is usually a team of two, the slicers carry out initial repairs to prevent the slice tissues from wrinkled; later, the size of the slicer is adjusted to the required micrometre thickness, which is then cut; it is then laid on the water surface with a tatter, which is used to level the tissue with water tension and temperature。
The person in charge of the sticker recovered the tissue with the film, which was then baked and dyed, and a pathological slice was completed。
Finally, the slices are sent to the diagnostic physician's office in the corresponding specialized areas of the diagnostic unit for observation, analysis and diagnosis under the microscope。
“we currently have 63 regular employees in the pathology section, of whom 31 are diagnostic doctors and 32 are technicians. There are many procedures for completing the entire pathology report, which requires the participation of more than a dozen people。
As science and technology develop, in addition to diagnosis through the observation of pathological slices under the microscope, the pathology department has many new technologies in place to improve the accuracy and accuracy of diagnosis. “if we are still unable to determine pathological results through microscopes, we will also have to carry out immunisation tests and molecular tests leading to an accurate pathological diagnosis of the patient.” lin xiaoyan explained that “it is essential to achieve individualized treatment, especially for oncology patients”
In recent years, the pathology section of shandong provincial hospital has actively explored the management of indoor quality control in projects such as hee dyeing, immunisation, arms law pcr, pathological diagnosis, etc., and has developed a variety of fluorescent field hybrid techniques (fish). The unit is based on dako lThe pd-l1 testing of immunotherapy markers was carried out on the nk48 platform, and new tests of the brca and lung cancer 10 genes were carried out on the basis of single and combined (9 genes). The implementation of this series of projects has not only increased the level of diagnosis of suspected cases in pathology, but has also provided important safeguards for clinical precision treatment of diseases。
