Did you pick the right group

Did you pick the right group

Did you pick the right group? If the antibodies are wrong or wrong, it's easy to get into a puddle that can't be sold or can't be reported to the client! Let's see what a professional antibody veteran does

It's mainly a three-dimensional approach: the requirements of the experiment, the recommendations of the operator, the safety of the factory

First, select the product from the requirements of the experiment。

A simple four-pronged question:

With this brief conversation with the teacher, we business people could probably recommend the right antibody for the teacher. In-depth knowledge also requires a number of factors: antibody sources, references, imports or national production, plant certification experiments, after-sale policies, sample expression, etc. The following is a basic concept of ihc and if:

Immunization tissue chemistry (ihc) and immunocellular chemistry (icc) are techniques used to locate and monitor antigen expression, the main rationale of which is to use the interaction between antigen surfaces and antibodies. Ihc uses tissue slices and icc uses cultured cells. Finally, ihc/icc is required to use molecular markers (fluorescent or dye) to obtain positive dye results. In short, the sample is fixed to preserve the cell integrity, then hatched with a closed reagent to cover the non-specific position where the antibody may combine, and lastly, with an anti- and anti-incubation sample and signal observations under the microscope。

If, immune fluorescent

Ihc-p, paraffin slices, which can be immunochemical and immuno-fluorescent can easily have high background

Ihc-fr, icy slices, immunochemical and immuno-fluorescent

Icc/if, applicable to cell samples, can be immunochemical or immuno-fluorescent

Ihc-fofr, injection of fixed frozen slices

In selecting antibodies, for different experimental methods such as if (immunization fluorescent), icc (cell chemical dyeing), ihc-fr (freeze tissue immunisation) and ihc-p (immunization of paraffins), we need to select the appropriate antibody product according to the specific needs and targets of the experiment。

Reasonable recommendation: brand name, literature references, brand advantages, and, more importantly, after-sales security

We're not groceries, but helpers who can advise your experiment

You don't believe me? Easy to enter

Maybe you can't sell antibodies from the literature! Because some manufacturers' after-sales policy is to sell samples and experiments they have tested, but it doesn't mean the antibody can't do the experiment, but it's not certified

Let's see what happens with the after-sales policy for all kinds of common antibodies

Maybe you're facing a situation where you can't sell, and you don't know what the scope of the if is...

Let's see their after-sales policy together

The importance of brand sales in selecting antibodies cannot be ignored, not only because of the importance of customer satisfaction and the smooth running of experiments, but also because of the reputational image of the brand。

It's not complete or correct. Please correct me! Thank you

It is hoped that this paper will provide practitioners with the opportunity to learn, help science teachers with antibody selection, and help with publicity for a wide range of antibody producers. With regard to the post-sales policy of other companies, i hope you can read the comments in the comment area and give us some help to salesmen and scientists

More biotech advice and experimental techniques

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