Unless you look like a monkey, it's a painful thing to count cells using a bloodball countboard. The point is inaccurate and repetitive. If there were multiple samples, it would be crazy. At that point, the automatic cell meter was created, resulting in a more efficient and accurate cell count。
There are several brands of cell counters on the market, which at first glance seem to be classic three-steps: top -- insert -- analyze. How to choose an ideal cell meter, a biographer to give you two strokes, so that you choose the right one, not the expensive one. First of all, think about which cells are to be counted, and what their size is; secondly, what kind of cell information you need and the flux level; how much space your lab is going to devote to the cell meter; and finally, how much money you're going to spend。
Last time we introduced two hot autocellometers. Today, the biographer shows you two tiny cell meters. Maybe, you want to take the cell meter to the super-purification table. At this point, the portable cell counter might be more appropriate for you. Both instruments use the principle of electrical resistance cell count, counting accurately and without the need for blue dyes。
Scepter handheld cell count (merkmirborg)
Merkmirebo launched the scepter handheld cell count last march, the only handheld automatic cell counter in the market today. One year later, scepter was upgraded to version 2. 0, further improving performance。
Scepter looks like a fluid transferer, but at the top there is a screen that guides users in cell count. An operator can automatically inhale a cell by pressing a light press, resulting in a complete histogram showing the diameter and volume of the cell, providing data on cell density and cell mass distribution, providing an easy quantitative means of controlling the health of the cell, the process taking only 14 seconds. By comparing histograms, you can easily detect changes in cell growth between cell cultures. Scepter's innovative form of liquid emulator design, which is small and flexible and can be counted directly by only one step, is “very convenient” for researchers who expect to be able to count multiple samples in a specific area of operation, such as ultra-purification table, and there is definitely a “jump-up” in the sense of use. Scepter was also successful in selecting one of the 10 major innovations in the life sciences 2010 early in the year。
Scepter has adopted the principle of electrical resistance to cell count (also known as the kurtian method), a core technology that has been centralized in sensor through fine microcircuit designs. In diluted cell suspensions, such as seep electrolytic solutions, interpolating sensor, internal and external electrodes on both sides of sensor's micro-route generate stable currents of electricity following connection to the power supply, diluting cell fluids flow from the outer side of the tube to the inner side of the small cavity, increasing electrical resistance within the small perforation zone, causing instant voltage changes to form pulse signals, pulse vibrations, larger cell sizes, more pulses and more cells, resulting in cell numbers and volume values, which are derived from cell diameters derived from cell sizes. So scepter can monitor the number of cells, their size and diameter。
Of course, as a result of the resistance method used, any particles that pass through a small hole are counted, both live cells, dead cells and cell fragments. Because the dead cells will have significant cell sizes, and scepter's results are shown in histograms, you can set the upper and lower limit of the values, as needed, so that cell fragments and most of the dead cells can be easily removed。
Two sensor: 40 μm and 60 μm are currently available for scepter handheld cell counters. This allows scepter to count 3-36 μm cells for mammalian cells, yeasts, algae and others. If you want to count smaller cells, you can choose 40 μm sensor and 60 μm sensor. The specific parameters are detailed in the table below. The one-time use of sensor is easy and quick to extract and analyse, saves the “transfer” steps, avoids problems such as cleaning and cross-pollution and further enhances the perception of use。
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Moxitm z minicell counter (orflo)
This mini cell meter was developed by the american orflo technologies. Although its name is unknown, it is also the developer of the above instrument. Thus, the two instruments are broadly the same in principle, using the kurtian principle, although different sizes, different consumption materials and different software。
Moxi z uses a small cassette for counting, each of which can accommodate two samples. The unique design allows samples to be sampled from both ends. When cassette is inserted, the instrument's "smart technology" is automatically detected and the cassette is tested as being completely new or used. For all samples, the same cassette is used。
It takes only eight seconds for each measurement to produce parameters such as cell size, cell count, etc. Orflo has also developed a patent algorithm to calculate a cell health index (moxivability index), which is highly relevant to recognized dye methods. Moxiz is able to count cells 2-34 μm in diameter and applies to mammalian cells, slabs, yeasts and others. The risk of missing cells was also avoided because the test floor was 2 μm. A sample of 75 μl is required for each measurement, with cell concentrations ranging from 3,000 to 2,500,000 cells/ml。
After the measurement, the screen is shown below. The figure includes not only the number and average size of cells, but also a complete histogram reflecting the distribution of cell sizes in the sample. By comparing histograms, you can easily detect cell changes between cell cultures. The instrument itself can store data on 500 samples. In addition, all data can be transmitted to computers via usb interface or bluetooth。
Moxi z's catalogue price in the united states is about $3,000, which is much cheaper than the $70,000 in cantons. However, it is more difficult to find agents in china at present. It is believed that the article will be introduced by the manufacturer shortly after its publication。
Biology point assessment: designed as a scepter liquid mover, which is light, allows sampling to be counted, but with a higher lower limit to detect cell concentrations. Moxi z detected a lower lower cell range to calculate the ratio of active to dead cells, but less portable and operational and more sample-intensive. As far as instruments are concerned, after-sales are important, and we have to consider the convenience of after-sale services。
Name
Scepter handheld cell count
Moxi z minicell counter
Manufacturers
Merkmireb
Orflo
Cell concentration
50,000–1,500,000 cells/ml
(40 μm sensor)
10,000-500,000 cells/ml
(60 metre sensor)
3,000 - 500,000 cells/ml
Cell diameter
3-18 μm (40 μm sensor)
6–36 mm (60 mm sensor)
2-34 mm
Sample volume
50 millimetres
75 ml
Analysis time
Take a sample of 500,000 cells/ml
40 meters sensor, 25 seconds
60 meters sensor, 14 seconds
8 seconds (concentration not provided)
Analysis parameters
Number of cells, size and diameter of cells
Number of cells, cell size, diameter size, cell health index
Flux
The dye
No need
No need
Annex
Scepter sensors
40 mm/ 60 mm
Moxi cassette
Dimensions
Long: 29 cm; width: 5. 5 cm
~19 x 10. 8 x 7 cm
(long x wide x high)
Weight
~0. 23 kg
~0. 65 kg
This parameter table is organized according to the manufacturer's data and is available for reference only. Biological tolerance is not notified unless the manufacturer changes。
(biology chattering)
Autocellometer selection guide (i)
Autocell counter selection guide (iii) - high flux
