Tomato is one of the main economic tree species grown traditionally in jiangsu province, and the direction of industrial development is gradually shifting towards the varieties of tomatoes of high commodity value and market demand. Among them, the “taqu” tomatoes have gained international common recognition for their high quality and are most popular in our markets. Tomatoes are grown mainly through the technique of indoctrination, so the breeding and indoctrination of seedlings is an essential basis for their promotion. However, the “takaki” and the country's traditional zakagi settlers are married and have poor sexual relations and are unable to survive at a later stage, and there are problems such as the low growth of seedlings and poor resistance to anthrax in jiangsu region, where the lack of zengsu seriously limits the development of the tomato industry. Susu1 has collected a field tomato resource from jiangsu province for the fruit tree biotechnology laboratory of nanjing agricultural university. In the initial screening of over 20 wild tomatoes in jiangsu province, soo-su1 demonstrated a remarkable growth, resilience and marriage to the "tachiu" tomatoes. As a result, soprano-1 can be one of the good materials for the nudity of the tomato tree and the aluminum, with great potential for application in the good breeding of new varieties of the tomatoes。
Permium generally reproduces sexually with live seeds, but is prone to genetic variability in future generations. On the one hand, non-consistent growth of seedlings affects the alignment of the tree with the mating pattern; on the other hand, future generations may lose their original qualities. The organizational culture technique, as an efficient non-sexist method of reproduction, has the characteristics of short-term reproduction cycles, efficient reproduction and free from seasonal constraints. In the case of tomatoes, it is also possible to address the poor availability of some seed varieties and the inefficient breeding of live seedlings. The level of research on the techniques for the training of tomatoes is slower than that of other fruit trees, but through a series of studies by national and international scholars, the techniques for the development of the tissue are now widely applied to the breeding of tomatoes. The proliferation of techniques such as tissue culture and the regeneration of leaf blades can lead to rapid reproduction while maintaining the quality of varieties and group consistency, address the problem of the lack of accompanimental nectar wood in tomato production, and provide technical support for the follow-up study of the genetic transformation and genetic function of soprano-1。
Purpose
Technical support for the rapid growth of its size is provided through the establishment of a fast-growing system of soprano-1 through tissue training and the induction of leaves for regeneration。
Methodology
The hibernation of soprano-1 is used as an implant for primary tissue training to identify its most suitable basic culture by comparing its growth state; the screening of the most suitable next-generation conditions of soprano-1 using combinations of 吲哚-3-acetic acid (iaa) and maize zt (zt) at different concentrations; and the screening of the most hormonal conditions for the reculture of sopranonate-1 using combinations of benzeneron tdz (tdz) and acetic acid (naa) at different concentrations. The three basic cultures are dkw, 1/2ms and (1/2n)ms。
Result
Screening of basic cultures
The hibernation sprout of sourium 1 began to develop after access to three primary cultures of 14d, of which 1/2-ms cultures had a faster growth. After the vaccination, the lowest incidence of hibernation was found in the dkw cultures, while the rate of growth of the 1/2ms culture was much different from that of the (1/2n) ms culture (table 1)。
The hibernation buds in the cultures grew to 1. 5 - 2. 0 cm at 30 d of the vaccination. Following the vaccination of 60d, the leaves of the various combinations were largely stretched, of which (1/2n) ms culture was the most dynamic, with the largest spread of the leaves, a strong leaf and a strong green leaf; 1/2 ms culture was green in the middle of the leaves, with internal rolls and partially blacked out in the leaves; the number of leaves in the dkw culture was relatively small, the leaves were yellowized, blackened and the base section was large (figure 1). Taken together, sourium 1 is the best growing plant in the basic culture base of ms (1/2n)。
Note: a. 1/2ms culture foundations; b.(1/2n)ms culture foundations; c. Dkw culture foundations。
Figure 1
Impact of different hormone combinations on intergenerational growth
The growth status of sulphate-1 seedlings in cultures in different hormone combinations is shown in figure 2, table 2. As can be seen from figure 2, table 2, iaa concentrations of 0. 10 mg/l (composite 4-6) were higher than 0. 05 mg/lia (composite 1-3). Zt concentrations were 1. 0 mg/l, with the best growing strains, with a strong leaf, fresh green and non-browning, and a smaller base wound; zt concentrations 0. 5 mg/l, with a slightly weaker plant, with light greening and partially yellowing of the leaves at iaa 0. 05 mg/l; and zt concentrations 3. 0 mg/l, with a much more bruised base, with severe browning at iaaa concentrations of 0. 10 mg/l, while iaaa concentrations were 0. 05 mg/l, with small and large leaves。
Figure 2 growth status of the soprano 1 relay group under different hormone combinations
In the area of breeding, the 0. 5 mg/l zt (assembly 1, 4) was significantly smaller, while the zt concentration was 1. 0 mg/l (cluster 2, 5) and 3. 0 mg/l (cluster 3, 6) were not significantly different. In sum, the growth effect of the sulphate 1 group seedlings under iaa 0. 1 mg/l+zt 1. 0 mg/l is best。
The effects of different hormone combinations on the induction and re-emergence of the tissues of the leaves
There is no significant difference in the induction rate of the sulphate 1 ion blades at all three naa concentrations, but the increase in tdz concentrations has increased significantly, with the highest induction rates of 90 per cent at tdz3. 0 mg/l, naa 0. 2 mg/l (table 3, figure 3)。
Note: the different lowercase letters in the same column indicate significant variations between groups (p)
Figure 3 leaf sorbonne no. 1 (a, b) and uncertain rejuvenation (c)
The occurrence of re-emergence was similar to that of re-emergence at tdz and naa concentrations, with the highest number of indeterminate sprouts occurring at tdz3. Mg/l. At the same tdz concentration, most of the combinations of different naa concentrations did not differ significantly, and only at tdz2. 0mg/l, naa 0. 2mg/l (cluster 5) had an indeterminate number significantly higher than naa 0. 3mg/l (cluster 8), indicating that excessive naa concentrations may inhibit the formation of indeterminate foliage tissues. In sum, the regenerative effects of the sulphate 1 ion blades under the conditions of tdz3. Mg/l and naa0. 2 mg/l are best, with a induction rate of 90. 0 per cent and an average of 3. 1 sproutings。
Conclusions
(a) the establishment of a fast-growing group system of soprano-1: (1/2 n) ms is the most suitable basic culture base; (1/2 n) ms + 0. 1 mg/l iaa+1. 0 mg/l zt is the most suitable condition for the next generation; and (1/2 n) ms + 3. 0 mg/l tdz + 0. 2 mg/l naa is the most suitable condition to induce the injury of leaves。
